Medical care

We have conducted a single oral administration toxicity study using rats for “Hydrogen NanoGAS^® Water ” and “Ozone NanoGAS^® Water “, which we provide as paid samples.

In conclusion, no rats died in either group,and drinking normal amounts of “Hydrogen NanoGAS^® Water ” and “Ozone NanoGAS^® Water ” was shown to be “non-toxic.

The following is a report from the testing organization.

Title of Examination

Single oral dose toxicity study using Hydrogen NanoGAS^® Water and Ozone NanoGAS^® Water rats

Study sites】 【Facilities where the study was conducted

GLP CERTIFICATION BODY

Purpose

To study the toxicity of a single oral dose of hydrogen NanoGAS^® water and ozone NanoGAS^® water in rats.

[Methods

Six-week-old female rats, fasted for approximately 18 hours prior to administration, were divided into groups of three rats each.

A single forced oral dose of hydrogen NanoGAS^® water and ozone NanoGAS^® water at a volume of 20 mL/kg was administered, followed by 14 days of live/dead, general condition, and weight measurements, and a necropsy at the end of the observation period.

A control group was also established in which tap water was administered in the same volume.

[Notation] [Notation

In the following table, “UFB Water A” refers to “Ozone NanoGAS^® Water,” “UFB Water B” refers to “Hydrogen NanoGAS^® Water,” and “Tap Water” refers to tap water in the control group.

(i) Life and death situations

No deaths occurred during the 14-day observation period.

Detailed results are shown in Table 1.

(2) General Condition

During the 14-day period of observation, no cases of abnormal behavior were observed.

Detailed results are shown in Table 2.

(iii) Weighing status

During the 14-day observation period, weight gain was observed, but there was no difference between the groups with respect to weight gain.

Detailed results are shown in Table 3.

(iv) Autopsy and histopathology

No gross abnormalities were observed in any of the animals on the external surface of the body or in any of the organs/tissues of the head, chest, and abdomen.

Detailed results are shown in Table 4.

There were no abnormalities in the large intestine (cecum, colon, or rectum) in any of the animals.

Detailed results are shown in Table 5.