{"id":9065,"date":"2021-04-12T14:16:52","date_gmt":"2021-04-12T05:16:52","guid":{"rendered":"https:\/\/symcnanoserpro.wpenginepowered.com\/document\/ozone-nanogas-water-report-on-primary-skin-irritation-test-results-using-rabbits\/"},"modified":"2023-11-20T18:01:16","modified_gmt":"2023-11-20T09:01:16","slug":"o3safety","status":"publish","type":"document","link":"https:\/\/nanogas.sym-biosis.co.jp\/en\/document\/o3safety\/","title":{"rendered":"Ozone NanoGAS\u00ae Water: Report on Primary Skin Irritation Test Results Using Rabbits"},"content":{"rendered":"<p><style>h2 { color: #333;    padding: 0.6em 0 0.5em;    margin: 1.5em 0 1.2em; border-top: 2px solid #47a5c1;    border-bottom: 1px solid #ccc; }<\/style><\/p>\n&#13;\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7874\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/6f5be9457447753d8b94242f97a3d3bf-1-600x400.png\" alt=\"\" width=\"600\" height=\"400\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/6f5be9457447753d8b94242f97a3d3bf-1-600x400.png 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/6f5be9457447753d8b94242f97a3d3bf-1-223x149.png 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/6f5be9457447753d8b94242f97a3d3bf-1.png 640w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/figure>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">We have conducted a skin primary irritation test on rabbits for ozone NanoGAS\u00ae water, which we currently provide as a paid sample.<\/p>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">As a result, we are pleased to report that the substance was determined to be &#8220;non-irritant,&#8221; showing no irritancy in either the naked eye or histopathological examination.<br\/>We would like to continue to conduct further verification and research with companies as a substance with the potential to have a highly safe antimicrobial function and cleaning function.<\/p>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">If your company would like to order samples for a fee, <strong><em><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/sample\/\" target=\"_blank\" rel=\"noopener\" data-type=\"page\" data-id=\"6325\"><u>please click<\/u><\/a><\/em><\/strong> Please see this link for a paid sample.<\/p>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">The following is a report from the testing organization.<\/p>&#13;\n\n<div class=\"wp-block-vk-blocks-alert alert alert-info\">\n<p>Test title : Primary skin irritation study in rabbits to ozone NanoGAS\u00ae water<br>Test site : GLP certified laboratory<\/p>\n<\/div>\n&#13;\n<p class=\"wp-block-paragraph\">For the purpose of studying the irritancy of a single dose of ozone ultrafine bubble water on the back skin of rabbits, a dose of 0.5 mL each was applied occluded to the back skin (healthy skin and damaged skin) of Japanese White female rabbits for 24 hours.<br\/>After 24, 48 and 72 hours of application, the formation of erythema\/crust and edema were observed grossly according to the Draize method, and histopathological examination was performed. The same dose of injectable water was also administered as a control substance.<\/p>&#13;\n&#13;\n<h2 class=\"wp-block-heading\">Skin irritation reaction (observed by naked eye)<\/h2>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">The results are shown in Table 1.<\/p>&#13;\n&#13;\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1285\" height=\"585\" class=\"wp-image-6528\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table.jpg\" alt=\"\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table.jpg 1285w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table-600x273.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table-1024x466.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table-223x102.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/N-I696_table-768x350.jpg 768w\" sizes=\"auto, (max-width: 1285px) 100vw, 1285px\" \/><\/a>&#13;\n<figcaption>Click to enlarge<\/figcaption>&#13;\n<\/figure>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">No irritative changes such as erythema\/crust and edema were observed in both healthy and damaged skin at the site of administration of either the test substance (ozone ultrafine bubble water) or the control substance (water for injection) throughout the observation period. The primary irritation index (P.I.I.) obtained from the results at 24, 48 and 72 hours after administration were all 0, and the product was evaluated as &#8220;no irritant&#8221;.<\/p>&#13;\n&#13;\n<h2 class=\"wp-block-heading\">histopathological examination<\/h2>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">The results are shown in Table 2 and Appendix 1.<\/p>&#13;\n&#13;\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"405\" class=\"wp-image-6527\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2-1024x405.jpg\" alt=\"\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2-1024x405.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2-600x238.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2-223x88.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2-768x304.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/2.jpg 1248w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>&#13;\n<figcaption>Click to enlarge<\/figcaption>&#13;\n<\/figure>&#13;\n&#13;\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"426\" class=\"wp-image-6524\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5-1024x426.jpg\" alt=\"\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5-1024x426.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5-600x249.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5-223x93.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5-768x319.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/5.jpg 1287w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>&#13;\n<figcaption>Click to enlarge<\/figcaption>&#13;\n<\/figure>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">Minor dermal cell infiltration was observed in 2\/3 of the cases in the test substance-treated healthy and damaged skin, and in 2\/3 of the cases in the control substance-treated healthy and damaged skin. However, since there was no difference in the quality and degree of lesions between the test and control substances, or between healthy and damaged skin, it was considered to be a change in reactivity to the administration of the test substance and was judged to be unrelated to the effect of the test substance administration.<\/p>&#13;\n&#13;\n<h2 class=\"wp-block-heading\">general situation<\/h2>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">The results are shown in Table 3.<\/p>&#13;\n&#13;\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"355\" class=\"wp-image-6526\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3-1024x355.jpg\" alt=\"\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3-1024x355.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3-600x208.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3-223x77.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3-768x266.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/3.jpg 1145w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">No abnormalities in general condition were observed in any of the animals throughout the observation period.<\/p>&#13;\n&#13;\n<h2 class=\"wp-block-heading\">Weight change<\/h2>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">The results are shown in Table 4.<\/p>&#13;\n&#13;\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"318\" class=\"wp-image-6525\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4-1024x318.jpg\" alt=\"\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4-1024x318.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4-600x186.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4-223x69.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4-768x239.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/04\/4.jpg 1133w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>&#13;\n<figcaption>Click to enlarge<\/figcaption>&#13;\n<\/figure>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">No decrease in body weight or other abnormalities were observed in any of the animals on the third day of administration.<\/p>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">Based on the above results, the ozone ultrafine bubble water was determined to be a &#8220;non-irritant&#8221; product, showing no irritancy in either gross or histopathological examinations.<\/p>&#13;\n\n<div class=\"wp-block-vk-blocks-button vk_button vk_button-align-wide\"><a class=\"vk_button_link btn btn-info btn-md\" role=\"button\" href=\"https:\/\/nanogas.sym-biosis.co.jp\/sample\/ozone\/\" target=\"_blank\" rel=\"noopener\" aria-pressed=\"true\"><span class=\"vk_button_link_txt\">Learn more about paid samples of ozone NanoGAS\u00ae water.<\/span><\/a><\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#13; We have conducted a skin primary irritation test on rabbits for ozone NanoGAS\u00ae water, which we currently provide as a paid sample. &#13; &#13; As a result, we are pleased to report that the substance was determined to be &#8220;non-irritant,&#8221; showing no irritancy in either the naked eye or histopathological examination.We would like to continue to conduct further verification and research with companies as a substance with the potential to have a highly safe antimicrobial function and cleaning function. &#13; &#13; If your company would like to order samples for a fee, please click Please see this link for a paid sample. &#13; &#13; The following is a report&#8230;<\/p>\n","protected":false},"featured_media":9659,"template":"","tags":[51],"documents":[60],"class_list":["post-9065","document","type-document","status-publish","has-post-thumbnail","hentry","tag-nanoga-validation-test","documents-test_results"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/document\/9065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/document"}],"about":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/types\/document"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/media\/9659"}],"wp:attachment":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/media?parent=9065"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/tags?post=9065"},{"taxonomy":"documents","embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/documents?post=9065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}