{"id":9057,"date":"2021-11-02T04:00:00","date_gmt":"2021-11-01T19:00:00","guid":{"rendered":"https:\/\/symcnanoserpro.wpenginepowered.com\/document\/hydrogen-nanogas-water-ozone-nanogas-water-single-oral-dose-toxicity-study-in-rats\/"},"modified":"2023-10-26T16:45:20","modified_gmt":"2023-10-26T07:45:20","slug":"nanogasnontoxic","status":"publish","type":"document","link":"https:\/\/nanogas.sym-biosis.co.jp\/en\/document\/nanogasnontoxic\/","title":{"rendered":"Hydrogen NanoGAS\u00ae Water, Ozone NanoGAS\u00ae Water: Single oral dose toxicity study in rats"},"content":{"rendered":"<style>h2 { color: #333;    padding: 0.6em 0 0.5em;    margin: 1.5em 0 1.2em; border-top: 2px solid #47a5c1;    border-bottom: 1px solid #ccc; }<\/style>\n\n\n<p>We have conducted a single oral administration toxicity study using rats for &#8220;Hydrogen NanoGAS<sup>\u00ae<\/sup> Water &#8221; and &#8220;Ozone NanoGAS<sup>\u00ae<\/sup> Water &#8220;, which we provide as paid samples.<\/p>\n\n\n\n<p><strong>In conclusion, no rats died in either group,<span class=\"has-inline-color has-vivid-red-color\">and drinking normal amounts of &#8220;Hydrogen NanoGAS<sup>\u00ae<\/sup> Water &#8221; and &#8220;Ozone NanoGAS<sup>\u00ae<\/sup> Water &#8221; was shown to be <span style=\"text-decoration: underline;\">&#8220;non-toxic<\/span><\/span>.<\/strong><\/p>\n\n\n\n<p>The following is a report from the testing organization.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>Title of Examination <\/p>\n\n\n\n<p>Single oral dose toxicity study using Hydrogen NanoGAS<sup>\u00ae<\/sup> Water and Ozone NanoGAS<sup>\u00ae<\/sup> Water rats<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>Study sites\u3011 \u3010Facilities where the study was conducted<\/p>\n\n\n\n<p>GLP CERTIFICATION BODY<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>Purpose<\/p>\n\n\n\n<p>To study the toxicity of a single oral dose of hydrogen NanoGAS<sup>\u00ae<\/sup> water and ozone NanoGAS<sup>\u00ae<\/sup> water in rats.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>[Methods <\/p>\n\n\n\n<p>Six-week-old female rats, fasted for approximately 18 hours prior to administration, were divided into groups of three rats each.<\/p>\n\n\n\n<p>A single forced oral dose of hydrogen NanoGAS<sup>\u00ae<\/sup> water and ozone NanoGAS<sup>\u00ae<\/sup> water at a volume of 20 mL\/kg was administered, followed by 14 days of live\/dead, general condition, and weight measurements, and a necropsy at the end of the observation period.<\/p>\n\n\n\n<p>A control group was also established in which tap water was administered in the same volume.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>[Notation] [Notation <\/p>\n\n\n\n<p>In the following table, &#8220;UFB Water A&#8221; refers to &#8220;Ozone NanoGAS<sup>\u00ae<\/sup> Water,&#8221; &#8220;UFB Water B&#8221; refers to &#8220;Hydrogen NanoGAS<sup>\u00ae<\/sup> Water,&#8221; and &#8220;Tap Water&#8221; refers to tap water in the control group.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"page-link-01\">(i) Life and death situations<\/h2>\n\n\n\n<p>No deaths occurred during the 14-day observation period.<\/p>\n\n\n\n<p>Detailed results are shown in Table 1.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"355\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52-1024x355.jpg\" alt=\"\" class=\"wp-image-7905\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52-1024x355.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52-600x208.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52-223x77.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52-768x266.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/0161352a3d2ab457dfa6640c52a8eb52.jpg 1536w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Table 1: Life and Death Situations<\/figcaption><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"page-link-02\">(2) General Condition<\/h2>\n\n\n\n<p>During the 14-day period of observation, no cases of abnormal behavior were observed.<\/p>\n\n\n\n<p>Detailed results are shown in Table 2.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"604\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734-1024x604.jpg\" alt=\"\" class=\"wp-image-7906\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734-1024x604.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734-600x354.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734-223x132.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734-768x453.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/02a26045f6d6ef88e94b9f8505014734.jpg 1536w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Table 2. general state of affairs<\/figcaption><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"page-link-03\">(iii) Weighing status<\/h2>\n\n\n\n<p>During the 14-day observation period, weight gain was observed, but there was no difference between the groups with respect to weight gain.<\/p>\n\n\n\n<p>Detailed results are shown in Table 3.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"341\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf-1024x341.jpg\" alt=\"\" class=\"wp-image-7907\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf-1024x341.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf-600x200.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf-223x74.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf-768x256.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/63a6631bb0826417be60b91a4e23ffdf.jpg 1536w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Table 3: Weighing Status<\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"page-link-04\">(iv) Autopsy and histopathology<\/h2>\n\n\n\n<p>No gross abnormalities were observed in any of the animals on the external surface of the body or in any of the organs\/tissues of the head, chest, and abdomen.<\/p>\n\n\n\n<p>Detailed results are shown in Table 4.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"418\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141-1024x418.jpg\" alt=\"\" class=\"wp-image-7909\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141-1024x418.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141-600x245.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141-223x91.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141-768x314.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/24782b0b0c3e6059cb98d0b8a59e9141.jpg 1536w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Table 4. autopsy results<\/figcaption><\/figure>\n<\/div>\n\n\n<p>There were no abnormalities in the large intestine (cecum, colon, or rectum) in any of the animals.<\/p>\n\n\n\n<p>Detailed results are shown in Table 5.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"585\" src=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb-1024x585.jpg\" alt=\"\" class=\"wp-image-7910\" srcset=\"https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb-1024x585.jpg 1024w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb-600x343.jpg 600w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb-223x127.jpg 223w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb-768x439.jpg 768w, https:\/\/nanogas.sym-biosis.co.jp\/wp-content\/uploads\/2021\/11\/286ea4dd8294d47e9506039ca5746ddb.jpg 1536w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Table 5. results of histopathological examination<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>We have conducted an oral toxicity study of NanoGAS Water in rats. The results are now available and we are pleased to report them here.<\/p>\n","protected":false},"featured_media":9657,"template":"","tags":[51],"documents":[60],"class_list":["post-9057","document","type-document","status-publish","has-post-thumbnail","hentry","tag-nanoga-validation-test","documents-test_results"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/document\/9057","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/document"}],"about":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/types\/document"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/media\/9657"}],"wp:attachment":[{"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/media?parent=9057"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/tags?post=9057"},{"taxonomy":"documents","embeddable":true,"href":"https:\/\/nanogas.sym-biosis.co.jp\/en\/wp-json\/wp\/v2\/documents?post=9057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}